RhD Specific Antibodies Are Not Detectable in HLA-DRB1 Mice Challenged with Human RhD Positive Erythrocytes

Figure 2

Immunization of standard laboratory mice with human RhD positive RBC induces RBC-reactive but not RhD-specific IgG as detected by flow cytometry. Conventional inbred and outbred mice were immunized with a single dose of 10⁸ RhD positive RBC (blood group A) (white bars) (C57BL/6 , DBA1/J , CFW(SW) , CD1(ICR) , NSA(CF-1) ). Untreated mice were used as negative controls (grey bars) (). (a) Antibodies reactive with human RhD positive RBC (blood group A). (b) Antibody binding to RhD positive RBC after adsorbing the sera with human RhD^- cells (blood group A). MFI: mean fluorescence intensity.

(a)

(b)