Thalassemia and Hemoglobin E in Southern Thai Blood Donors

Figure 1

Representative 1.5% agarose gel electrophoresis of the amplified PCR products for characterizing 𝛼-globin gene deletion types by multiplex gap PCR. The 2 kb, 1.8 kb, 1.6 kb, 1.4 kb and 1.2 kb represent 3.7 kb deletion fragment (-𝛼^3.7), normal fragment (𝛼𝛼), 4.2 kb deletion fragment (-𝛼^4.2), SEA type deletion fragment (--^SEA), and THAI type deletion fragment (--^THAI), respectively. The M represents the 1 kb DNA ladder. Lanes 1–4 (positive controls) are genotypes as follows: -α ^3.7/α α, -α ^4.2/α α, --^SEA/𝛼𝛼, and --^THAI/𝛼𝛼, respectively. Lanes 5, 6, 8, 9, and 11–13 are normal 𝛼-globin genotype (𝛼𝛼/𝛼𝛼). Lanes 7, 10, and 15 are heterozygous α-thalassemia 1 (--^SEA/α α). Lane 14 is homozygous 𝛼-thalassemia 2 (-𝛼^3.7/-𝛼^3.7).