Characterization of IXINITY® (Trenonacog Alfa), a Recombinant Factor IX with Primary Sequence Corresponding to the Threonine-148 Polymorph

Figure 1

SDS-PAGE was performed using a NuPAGE 12% gel and 4x LDS sample buffer from Life Technologies (Grand Island, NY). Reduced lanes were treated with β-mercaptoethanol to 5% of total sample volume. 2 μg of protein was loaded in each lane. Gels were stained with Coomassie Blue. Lanes are (from left to right), SeeBlue Plus 2 standard, lot 1, lot 2, lot 3, lot 4, lot 1 reduced, lot 2 reduced, lot 3 reduced, lot 4 reduced, and SeeBlue Plus 2 standard. Molecular weights corresponding to the standards are shown.