Characterization of IXINITY® (Trenonacog Alfa), a Recombinant Factor IX with Primary Sequence Corresponding to the Threonine-148 Polymorph

Figure 2

Gel filtration chromatography was performed using a TSK-Gel G3000SWXL column run at 22°C at a flow rate of 0.5 mL min⁻¹. The column was run in 50 mM Tris, 200 mM NaCl, and pH 7.5. 40 μg of total protein was loaded on the column per run. Absorbance at 280 nm is plotted against time in minutes. The upper tracing shows trenonacog alfa run in the absence of calcium. The lower tracing shows trenonacog alfa run in the presence of 5 mM CaCl₂.

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