Deletional Alpha-Thalassemia Alleles in Amazon Blood Donors

Table 1

Primers used for detection of common deletional determinants of alpha-thalassemia.

Primer code	5′-3′ sequence	Fragment size (base pairs)
LIS1-F	G T C G T C A C T G G C A G C G T A G A T C	∼2503
LIS1-R	G A T T C C A G G T T G T A G A C G G A C T G
α2^3.7F	C C C C T C G C C A A G T C C A C C C	∼2022
α2 R	A G A C C A G G A A G G G C C G G T G
α2^3.7F	C C C C T C G C C A A G T C C A C C C	∼1800
^3.7/20.5R	A A A G C A C T C T A G G G T C C A G C G
SEA-F	C G A T C T G G G C T C T G T G T T C T C	∼1350
SEA-R	A G C C C A C G T T G T G T T C A T G G C
^4.2F	G G T T T A C C C A T G T G G T G C C T C	∼1630
^4.2R	C C C G T T G G A T C T T C T C A T T T C C C
^20.5F	G C C C A A C A T C C G G A G T A C A T G	∼1000
^3.7/20.5R	A A A G C A C T C T A G G G T C C A G C G
FIL-F	T G C A A A T A T G T T T C T C T C A T T C T G TG	∼1170
FIL-R	A T A A C C T T T A T C T G C C A C A T G T A G C
MED-F	T A C C C T T T G C A A G C A C A C G T A C	∼807
MED-R	T C A A T C T C C G A C A G C T C C G A C