Osteogenic Potential of Multipotent Adult Progenitor Cells for Calvaria Bone Regeneration

Figure 2

In vitro assessment of osteogenic differentiation of MAPCs on the HG material-coated dishes. Immunohistochemical staining with osteocalcin (a) and collagen type I (b) antibodies against MAPCs cultured on the HG coated culture plate (bottom) and without a coating as control (top). Mineral formation was detected by Alizarin Red S staining (c) after culturing MAPCs with osteogenic media on the coated dish (middle), no coating as control (top), and coated dish without MAPCs (bottom). Microscopic images further confirmed the ability of mineralization by MAPCs after 42 days of osteogenic differentiation.

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