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Advances in Medicine
Volume 2016, Article ID 4243784, 3 pages
http://dx.doi.org/10.1155/2016/4243784
Research Article

Assessment of Newly Proposed Clinical Criteria to Identify HNF1A MODY in Patients with an Initial Diagnosis of Type 1 or Type 2 Diabetes Mellitus

Department of Metabolic Diseases, Jagiellonian University Medical College, Jagiellonian University, 15 Kopernika Street, 31-501 Krakow, Poland

Received 23 October 2015; Accepted 11 January 2016

Academic Editor: Elizabeth Barrett-Connor

Copyright © 2016 Malgorzata Grzanka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The most common form of maturity-onset diabetes of the young (MODY) is caused by mutations in the hepatocyte nuclear factor 1A (HNF1A) gene. However, most HNF1A mutation-carriers are initially misdiagnosed with type 1 (T1DM) or type 2 (T2DM) diabetes mellitus; hence, they often receive nonoptimal treatment. The aim of our study was to test newly proposed clinical criteria for the identification of HNF1A MODY in patients with a diagnosis of T1DM or T2DM. To achieve this, the following criteria to preselect patients for screening were used: for T1DM: TDIR (total daily insulin requirement) > 0.3 IU of insulin/kg and the percentage of basal insulin > 30% of TDIR; for T2DM: sulphonylurea- (SU-) based oral treatment (monotherapy or combined with Metformin) > 15 years and BMI < 30 kg/m2. We reviewed the clinical data of 140 patients with T1DM and 524 clinically diagnosed with T2DM. On the basis of these criteria, we found a HNF1A mutation in 1 out of 2 individuals with a diagnosis of T1DM and 1 out of 11 selected individuals with a diagnosis of T2DM. We believe that the simplicity of the proposed criteria might prove useful in clinical practice, as an alternative to more time-consuming classical diagnostic techniques.

1. Introduction

The most common form of maturity-onset diabetes of the young (MODY) is caused by mutations in the hepatocyte nuclear factor 1A (HNF1A) gene [1, 2]. It is well established that patients with HNF1A MODY are very sensitive to sulphonylureas (SU) and can be taken off insulin treatment after the proper molecular diagnosis [1, 2]. However, most patients with this type of monogenic diabetes are initially misdiagnosed with either type 1 (T1DM) or type 2 (T2DM) diabetes mellitus; hence, they often receive nonoptimal treatment [3]. The effective selection of “T1DM” and “T2DM” patients for molecular testing for the HNF1A MODY mutation does present some challenges. It is usually based on clinical criteria suggested by Ellard et al. [2]. For HNF1A MODY they include young-onset diabetes, being non-insulin-dependent outside the normal honeymoon period, family history of diabetes, the absence of pancreatic islet autoantibodies, glycosuria at blood glucose levels <10 mmol/L, marked sensitivity to sulfonylureas, and no marked obesity or evidence of insulin resistance [2].

However, a recent retrospective analysis of a large pediatric cohort showed that only a small portion of MODY subjects were properly diagnosed in spite of having negative pancreatic autoantibodies, preserved endogenous insulin production, or strong family history of diabetes in multiple generations [4]. Biochemical biomarkers are also of limited value [2]. High sensitivity CRP (hsCRP) seemed to be one of the most promising. However recent data suggest that while being a good marker to make differential diagnosis between HNF1A MODY and T2DM, it brings no additional value to differentiate between patients with established diagnosis of HNF1A MODY and individuals with a clinical history suggestive of HNF1A MODY, but not confirmed genetically [5].

Aim of the Study. The aim of our study was to test newly proposed clinical criteria for selecting patients with an initial clinical diagnosis of either T1DM or T2DM for HNF1A MODY molecular testing.

2. Material and Methods

Subjects diagnosed with either T1DM or T2DM were preselected and ascertained as previously defined [6, 7]. All patients underwent molecular testing at the Department of Metabolic Diseases, Medical College, Jagiellonian University, Krakow, Poland [8]. The study was approved by the Bioethical Committee of Jagiellonian University.

2.1. Differential Diagnosis: T1DM versus HNF1A MODY

To establish the clinical criteria for preselecting individuals diagnosed with T1DM for HNF1A molecular testing, we performed a retrospective analysis of patients with an established HNF1A MODY diagnosis (131 patients, 46 males and 85 females). We found that before genetic testing 40 patients had been diagnosed and treated as T1DM patients. Based on the characteristics of these 40 misdiagnosed patients, we established new criteria for further molecular testing for HNF1A mutations. We subsequently reviewed the clinical data of 140 patients diagnosed with T1DM to preselect individuals meeting our newly established criteria. The diagnosis of T1DM was based on clinical criteria; autoantibodies were not determined.

3. Results

3.1. T1DM versus HNF1A

Analysis of the clinical data of 40 HNF1A mutation-carriers, who had been treated as T1DM before MODY diagnosis, showed that, on average, their total daily insulin dose was 0.28 j/kg ± 0.11 j (range: 0.19–0.34 j/kg). The data concerning the percentage of basal insulin versus TDIR was available for only six individuals and its median was equal to  j (range: 26–33%). On the basis of these data, we established the cut-off point for potential MODY at 0.3 IU of insulin per kg and the percentage of basal insulin being >30% of TDIR. As reported earlier, the mean insulin requirement per kg of body mass for T1DM patients was 0.69 IU/kg, whereas the percentage of basal insulin was 42.6% [9].

Of the 140 patients with an initial T1DM diagnosis, based on the aforementioned criteria, two individuals were selected for further molecular testing by Sanger sequencing. A previously described diabetes-related mutation in exon 4 (P291fsinsC) was found in one individual [1]. Interestingly, the maternal family history of this patient was negative for diabetes; however, no data on the paternal branch of the family was available.

3.2. T2DM versus HNF1A

To select subjects from the T2DM cohort for HNF1A gene sequencing, the following criteria were used: SU-based oral treatment (SU in monotherapy or combined with Metformin) for >15 years and a body mass index < 30 kg/m2. We analyzed the medical records of 524 patients clinically diagnosed with T2DM to preselect individuals for genetic screening for the HNF1A MODY mutation. Based on suggested clinical criteria, we selected 14 subjects from the T2DM patient cohort; however, only 11 were available for testing (9% of selected T2DM cases). In one of these 11 patients, a previously undescribed mutation was identified: a deletion of amino acids 1283–1309 of the HNF1A gene. Since this deletion is relatively large (1.3% of exon 7) and is located in the carboxyl-terminal transactivation domain, which is crucial for HNF1A function, it is highly probably that this is a causative variant of diabetes in this subject [10]. Retrospective analysis of the clinical data of this patient revealed positive family history for diabetes, which is suggestive of an autosomal-dominant mode of inheritance. Unfortunately, other family members were not available for segregation analysis.

4. Discussion

Misdiagnosis of monogenic diabetes has an impact both on glycemic control and on quality of life of affected individuals [1]. There is hope that, with improved accessibility of next generation sequencing (NGS), the number of patients correctly diagnosed with MODY will increase substantially; however, at this point in time, access to this high-throughput methodology is still limited [11]. Here, we propose new clinical criteria for preselection of patients initially diagnosed with either T1DM or T2DM for genetic testing for the identification of mutations in HNF1A. As there were some earlier reports suggesting that de novo MODY mutations might be more frequent than previously considered, we did not include family history as part of our criteria [12]. Of note, in our study, the HNF1A mutation-carrier identified among patients with a clinical diagnosis of T1DM was indeed negative for diabetes in his families. Interestingly, in a recent study involving patients with an initial diagnosis of T1DM, which used three traditional criteria (negative pancreatic autoantibodies, sustained endogenous insulin production, and strong family history of diabetes in multiple generations), only one of 58 patients was identified to be a HNF1A mutation-carrier [13].

As for differential diagnosis between T2DM and HNF1A MODY, the success rate based on our proposed preselection criteria was relatively small (1 out of 11). This may mean that clinical criteria to identify HNF1A mutation-carriers should be probably more restrictive. Testing them would require much larger group of patients to come up with sufficient number of individuals meeting those narrow, more restrictive criteria.

In general, due to relatively small sample size, our study should be considered as a preliminary one. Furthermore, the assessment of diagnostic sensitivity and specificity of our criteria would require sequencing, preferentially NGS, to be performed in the entire initial cohort of T1DM and T2DM subjects.

5. Conclusions

We believe that criteria proposed in the present study might be useful for clinicians, as an alternative to classical criteria, to select T1DM and T2DM patients who should undergo molecular testing for mutations in HNF1A. Though our criteria show potential, further research and validation are required.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

References

  1. C. Pihoker, L. K. Gilliam, S. Ellard et al., “Prevalence, characteristics and clinical diagnosis of maturity onset diabetes of the young due to mutations in HNF1A, HNF4A, and glucokinase: results from the SEARCH for diabetes in youth,” Journal of Clinical Endocrinology and Metabolism, vol. 98, no. 10, pp. 4055–4062, 2013. View at Publisher · View at Google Scholar · View at Scopus
  2. S. Ellard, C. Bellanné-Chantelot, and A. T. Hattersley, “Best practice guidelines for the molecular genetic diagnosis of maturity-onset diabetes of the young,” Diabetologia, vol. 51, no. 4, pp. 546–553, 2008. View at Publisher · View at Google Scholar
  3. M. A. Stone, J. Camosso-Stefinovic, J. Wilkinson, S. de Lusignan, A. T. Hattersley, and K. Khunti, “Incorrect and incomplete coding and classification of diabetes: a systematic review,” Diabetic Medicine, vol. 27, pp. 491–497, 2010. View at Google Scholar
  4. C. Chambers, A. Fouts, F. Dong et al., “Characteristics of maturity onset diabetes of the young in a large diabetes center,” Pediatric Diabetes, 2015. View at Publisher · View at Google Scholar
  5. C. Bellanné-Chantelot, J. Coste, C. Ciangura et al., “High-sensitivity C-reactive protein does not improve the differential diagnosis of HNF1A-MODY and familial young-onset type 2 diabetes: a grey zone analysis,” Diabetes & Metabolism, 2015. View at Publisher · View at Google Scholar
  6. N. Nowak, J. Hohendorff, I. Solecka et al., “Circulating ghrelin level is higher in HNF1A–MODY and GCK–MODY than in polygenic forms of diabetes mellitus,” Endocrine, vol. 50, no. 3, pp. 643–649, 2015. View at Publisher · View at Google Scholar
  7. N. Nowak, M. Szopa, G. Thanabalasingham et al., “Cystatin C is not a good candidate biomarker for HNF1A-MODY,” Acta Diabetologica, vol. 50, no. 5, pp. 815–820, 2013. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Szopa, G. Osmenda, G. Wilk et al., “Intima-media thickness and endothelial dysfunction in GCK and HNF1A-MODY patients,” European Journal of Endocrinology, vol. 172, no. 3, pp. 277–283, 2015. View at Publisher · View at Google Scholar
  9. B. Matejko, M. Grzanka, B. Kieć-Wilk, M. T. Malecki, and T. Klupa, “Clinical factors affecting the perception of hypoglycemia in type 1 diabetes patients treated with personal insulin pumps,” Annals of Agricultural and Environmental Medicine, vol. 20, no. 1, pp. 152–154, 2013. View at Google Scholar · View at Scopus
  10. E. Jeannot, L. Mellottee, P. Bioulac-Sage et al., “Spectrum of HNF1A somatic mutations in hepatocellular adenoma differs from that in patients with MODY3 and suggests genotoxic damage,” Diabetes, vol. 59, no. 7, pp. 1836–1844, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. A. L. Gloyn, J. H. Faber, D. Malmodin et al., “Metabolic profiling in maturity-onset diabetes of the young (MODY) and young onset type 2 diabetes fails to detect robust urinary biomarkers,” PLoS ONE, vol. 7, no. 7, Article ID e40962, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. J. Stanik, P. Dusatkova, O. Cinek et al., “De novo mutations of GCK, HNF1A and HNF4A may be more frequent in MODY than previously assumed,” Diabetologia, vol. 57, no. 3, pp. 480–484, 2014. View at Publisher · View at Google Scholar · View at Scopus
  13. R. G. Gandica, W. K. Chung, L. Deng, R. Goland, and M. P. Gallagher, “Identifying monogenic diabetes in a pediatric cohort with presumed type 1 diabetes,” Pediatric Diabetes, vol. 16, no. 3, pp. 227–233, 2015. View at Publisher · View at Google Scholar · View at Scopus