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Advances in Medicine
Volume 2016, Article ID 4985745, 7 pages
http://dx.doi.org/10.1155/2016/4985745
Research Article

Evaluation of HLA-G 14 bp Ins/Del and +3142G>C Polymorphism with Susceptibility and Early Disease Activity in Rheumatoid Arthritis

1Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran
2Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran
3Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran
4Department of Internal Medicine, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran 19857-17443, Iran

Received 1 April 2016; Revised 27 June 2016; Accepted 20 July 2016

Academic Editor: Maja Krajinovic

Copyright © 2016 Mohammad Hashemi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose/Background. Mounting evidence designates that HLA-G plays a role in the regulation of inflammatory processes and autoimmune diseases. There are controversial reports concerning the impact of HLA-G gene polymorphism on rheumatoid arthritis (RA). This study was aimed at examining the impact of 14 bp ins/del and +3142G>C polymorphism with susceptibility and early disease activity in RA patients in a sample of the Iranian population. Methods. This case-control study was done on 194 patients with RA and 158 healthy subjects. The HLA-G rs1063320 (+3142G>C) and rs66554220 (14 bp ins/del) variants were genotype by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFP) and PCR method, respectively. Results. The HLA-G +3142G>C polymorphism significantly decreased the risk of RA in codominant (OR = 0.61, 95% CI = 0.38–0.97, , GC versus GG; OR = 0.36, 95% CI = 0.14–0.92, , CC versus GG), dominant (OR = 0.56, 95% CI = 0.36–0.87, , GC + CC versus GG), and allele (OR = 0.58, 95% CI = 0.41–0.84, , C versus G) inheritance models tested. Our finding did not support an association between HLA-G 14 bp ins/del variant and risk/protection of RA. In addition, no significant association was found between the polymorphism and early disease activity. Conclusion. In summary, our results showed that HLA-G +3142G>C gene polymorphism significantly decreased the risk of RA in a sample of the Iranian population.