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Advances in Medicine
Volume 2016, Article ID 9242031, 6 pages
http://dx.doi.org/10.1155/2016/9242031
Research Article

Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit

1Department of Microbiology, Faculty of Science, Islamic Azad University, Varamin-Pishva Branch, Tehran, Iran
2Department of Medical Parasitology and Mycology, Kurdistan University of Medical Sciences, P.O. Box 14155-6446, Sanandaj, Iran
3Division of Molecular Biology, Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4Department of Microbiology, Faculty of Science, Islamic Azad University, North Tehran Branch, Tehran, Iran
5Department of Medical Mycology, Jondishapour University of Medical Sciences, Ahvaz, Iran
6Department of Medical Mycology, Tarbiat Modares University, Tehran, Iran
7Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Received 20 April 2016; Revised 11 June 2016; Accepted 15 June 2016

Academic Editor: Anastasia Kotanidou

Copyright © 2016 Elham Baghdadi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. Biofilms formed by Candida species which associated with drastically enhanced resistance against most antimicrobial agents. The aim of this study was to identify and determine the antifungal susceptibility pattern of Candida species isolated from endotracheal tubes from ICU patients. Methods. One hundred forty ICU patients with tracheal tubes who were intubated and mechanically ventilated were surveyed for endotracheal tube biofilms. Samples were processed for quantitative microbial culture. Yeast isolates were identified to the species level based on morphological characteristics and their identity was confirmed by PCR-RFLP. Antifungal susceptibility testing was determined according to CLSI document (M27-A3). Results. Ninety-five strains of Candida were obtained from endotracheal tubes of which C. albicans (; 35.7%) was the most frequently isolated species followed by other species which included C. glabrata (; 25.2%), C. parapsilosis (; 16.8%), C. tropicalis (; 12.6%), and C. krusei (; 9.4%). The resulting MIC90 for all Candida species were in increasing order as follows: caspofungin (0.5 μg/mL); amphotericin B (2 μg/mL); voriconazole (8.8 μg/mL); itraconazole (16 μg/mL); and fluconazole (64 μg/mL). Conclusion. Candida species recovered from endotracheal tube are the most susceptible to caspofungin.