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Volume 2010, Article ID 625919, 7 pages
Research Article

Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency

1Hematology Laboratory, Hospital Galdakao—Usansolo, Galdakao, 48960 Vizcaya, Spain
2Department of Pharmacology and Physiology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain

Received 20 December 2009; Accepted 31 March 2010

Academic Editor: Fernando Ferreira Costa

Copyright © 2010 Eloísa Urrechaga et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Reticulocyte hemoglobin content (CHr) and percentage of hypochromic cells (%Hypo) are restricted to the Siemens analysers. The aims of the study were to investigate the correlation of Red cells size factor (RSf) and low Hemoglobin density (LHD%), reported by Beckman-Coulter analysers, with CHr and %Hypo in the assessment of iron status in the presence of inflammation. 381 samples were run on both LH 750 (Beckman-Coulter) and Advia 2120 (Siemens) analysers. Correlation between parameters were calculated and the diagnostic performance of the new parameters was assessed. Results. Correlation between RSf and CHr, 𝑟 = 0 . 8 5 . ROC curve analysis for RSf in the diagnosis of iron restricted erythropoiesis defined as CH r < 2 8 pg: AUC 0.983; Cutoff 91.1%; Sensitivity 98.8%; Specificity 89.6% Correlation between LHD% and %Hypo, 𝑟 = 0 . 8 6 9 . ROC curve analysis for LHD% in the diagnosis of iron deficiency defined by %Hypo > 5 %: AUC 0.954; Cut off 6.0%; Sensitivity 96.6%; Specificity 83.2% Conclusions. RSf and LHD% could be reliable parameters for the study of iron metabolism status.