Correction of Fanconi Anemia Group C Hematopoietic Stem Cells Following Intrafemoral Gene Transfer
Figure 4
Functional correction of BM progenitors following LV-mediated FancC-EPFP gene transfer. CFC analysis of BM cells from IF-injected mice at four months following injection (a) and following chronic MMC treatments (b). Percent CFC numbers with MMC compared to cultures without MMC. Controls represent noninjected WT or mice. Data represent the obtained from at least 3 mice in each group each done in triplicates. . (c) and (d) Representative cytometric profiles of FancC-EGFP expression in BM cells from IF-injected mice at four months following injection (c) and following chronic MMC treatment (d). (e) and (f) Representative cytometric profiles of FancC-EGFP expression in Sca1-positive BM cells from IF-injected mice at four months following injection (e) and following chronic MMC treatment (f).
