Correction of Fanconi Anemia Group C Hematopoietic Stem Cells Following Intrafemoral Gene Transfer
Figure 7
Prevention of BM failure following IF gene transfer into mice with BM aplasia. (a) FancC-EGFP transgene expression in peripheral blood cells of preconditioned IF-injected wild type (WT), , and mice that received chronic MMC treatments at 7 weeks following IF-injection as a function of time. The arrow represents the start of weekly MMC injections (0.3 mg/kg). Each dot represents the FancC-EGFP expression of an individual IF-injected mouse. Horizontal line: mean value. (b) Representative cytometric profiles of FancC-EGFP expression in peripheral blood cell lineages from preconditioned IF-injected mice at 15 weeks following weekly MMC treatment. (c) Survival of preconditioned IF-injected () and () mice following MMC treatments. Control mice represent uninjected mice treated weakly with MMC (). (d) Representative histological appearances of the sternum from preconditioned and IF-injected mice at four months following IF injections (no treatment) and at 15 weeks following weekly MMC treatment ( MMC). Hematoxylin-eosin staining: original magnification 200.
