The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells
Figure 3
Effect of Epo on the oxidative stress of RBC and platelets from thalassemic patients. Diluted blood samples were untreated with or without 1 U/ml Epo for 3 hrs at 37°C, then stimulated (a, b) or not (c, d) with 1 mM H2O2 for 15 min, and assayed for ROS. RBC (e) and platelets (f) treated with or without Epo were also assayed for GSH. The results, presented as the average () of the mean fluorescence index (MFI) ± SD, show a decrease in ROS () and an increase in GSH () following Epo treatment.
