Diagnosis of Fanconi Anemia: Mutation Analysis by Next-Generation Sequencing

Figure 1

Specifications of 11 DNA libraries pooled and sequenced on one flow cell lane of an Illumina GAIIx. Numbers on top of the bars indicate the average sequencing depth obtained for the individual samples. Blue bars indicate total number of unique reads, red bars indicate reads that fall on target of the bait design, green bars indicate reads that fall in target regions covered with at least 20x depth. Sample 4 is a mix of genomic DNA from two individuals 4a and 4b.