Reduced PKC Activity Induces Senescent Phenotype in Erythrocytes
Figure 2
Modulation of PKC α localization and activity upon PMA treatment. (a) Activity of PKC in the presence of Ca2+ and lipid shows 10–30% reduction in PMA-treated group. Units of activity (a.u.) are arbitrary values assigned by the graphic tool. (b) Western blots of erythrocyte cytosolic and membrane proteins stained with anti-PKC α antibody (represented by N2 and N3) show a signal around 77 kDa in the cytosol of all treatment groups but in the membrane fractions of only the PMA-treated erythrocytes.
