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Anemia
Volume 2012 (2012), Article ID 707402, 12 pages
http://dx.doi.org/10.1155/2012/707402
Clinical Study

Cobalamin and Folic Acid Status in Relation to the Etiopathogenesis of Pancytopenia in Adults at a Tertiary Care Centre in North India

1Department of Medicine, Maulana Azad Medical College and Associated Hospitals, Bahadur Shah Zafar Marg, New Delhi 110002, India
2Department of Pathology, Maulana Azad Medical College and Associated Hospitals, Bahadur Shah Zafar Marg, New Delhi 110002, India
3Department of Ocular Pharmacology, Dr. Rajendra Prasad Eye Centre, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India

Received 20 October 2011; Revised 6 December 2011; Accepted 6 December 2011

Academic Editor: Bruno Annibale

Copyright © 2012 M. Premkumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. N. S. Young, “Aplastic anemia, myelodysplasia, and related bone marrow failure syndromes,” in Harrison’s Principles of Internal Medicine, D. L. Kasper, E. Braunwald, and A. S. Fauci, Eds., pp. 663–671, McGraw Hill, 17th edition, 2008. View at Google Scholar
  2. E. C. Guinan and A. Shimamura, “Acquired and inherited aplastic anemia syndromes,” in Wintrobes’s Clinical Hematology, pp. 1398–1419, Lippincott, Williams and Wilkins, 11th edition, 2004. View at Google Scholar
  3. M. B. Satterfield, L. T. Sniegoski, K. E. Sharpless et al., “Development of a new standard reference material: SRM 1955 (homocysteine and folate in human serum),” Analytical and Bioanalytical Chemistry, vol. 385, no. 3, pp. 612–622, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. P. M. Ueland, Ø. Midttun, A. Windelberg, A. Svardal, R. Skålevik, and S. Hustad, “Quantitative profiling of folate and one-carbon metabolism in large-scale epidemiological studies by mass spectrometry,” Clinical Chemistry and Laboratory Medicine, vol. 45, no. 12, pp. 1737–1745, 2007. View at Publisher · View at Google Scholar · View at Scopus
  5. C. M. Pfeiffer, Z. Fazili, L. McCoy, M. Zhang, and E. W. Gunter, “Determination of folate vitamers in human serum by stable-isotope-dilution tandem mass spectrometry and comparison with radioassay and microbiologic assay,” Clinical Chemistry, vol. 50, no. 2, pp. 423–432, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. O. Heudi, T. Kilinç, P. Fontannaz, and E. Marley, “Determination of Vitamin B12 in food products and in premixes by reversed-phase high performance liquid chromatography and immunoaffinity extraction,” Journal of Chromatography A, vol. 1101, no. 1-2, pp. 63–68, 2006. View at Publisher · View at Google Scholar · View at Scopus
  7. N. Anderssen, “The activity of lactate dehydrogenase in megaloblastic anemia,” Scandinavian Journal of Haematology, vol. 1, pp. 212–219, 1964. View at Google Scholar
  8. P. M. Emerson and J. H. Wilkinson, “Lactate dehydrogenase in the diagnosis and assessment of response to treatment of megaloblastic anaemia,” British Journal of Haematology, vol. 12, no. 6, pp. 678–688, 1966. View at Google Scholar · View at Scopus
  9. N. Varma and S. Dash, “A reappraisal of underlying pathology in adult patients presenting with pancytopenia,” Tropical and Geographical Medicine, vol. 44, no. 4, pp. 322–327, 1992. View at Google Scholar · View at Scopus
  10. K. Khodke, S. Marwah, G. Buxi, R. B. Yadav, and N. K. Chaturvedi, “Bone marrow examination in cases of pancytopenia,” Journal, Indian Academy of Clinical Medicine, vol. 2, pp. 55–59, 2001. View at Google Scholar
  11. G. Santra and B. K. Das, “A cross-sectional study of the clinical profile and aetiological spectrum of pancytopenia in a tertiary care centre,” Singapore Medical Journal, vol. 51, no. 10, pp. 806–812, 2010. View at Google Scholar · View at Scopus
  12. V. Tilak and R. Jain, “Pancytopenia—a clinico-hematologic analysis of 77 cases,” Indian Journal of Pathology and Microbiology, vol. 42, no. 4, pp. 399–404, 1999. View at Google Scholar · View at Scopus
  13. R. Kumar, S. P. Kalra, H. Kumar, A. C. Anand, and H. Madan, “Pancytopenia—a six year study,” Journal of Association of Physicians of India, vol. 49, pp. 1078–1081, 2001. View at Google Scholar · View at Scopus
  14. J. M. Khunger, S. Aruselvi, U. Sharma, S. Ranga, and V. H. Talib, “Pancytopenia—a clinicohematological study of 200 cases,” Indian Journal of Pathology and Microbiology, vol. 45, no. 3, pp. 375–379, 2002. View at Google Scholar
  15. H. Heimpel, “Incidence of aplastic anemia: the relevance of diagnostic criteria,” Blood, vol. 70, no. 6, pp. 1718–1721, 1987. View at Google Scholar · View at Scopus
  16. D. G. Savage, R. H. Allen, I. T. Gangaidzo et al., “Pancytopenia in Zimbabwe,” American Journal of the Medical Sciences, vol. 317, no. 1, pp. 22–32, 1999. View at Google Scholar · View at Scopus
  17. O. Ishtiaq, H. Z. Baqai, F. Anwer, and N. Hussain, “Patterns of pancytopenia patients in a general medical ward and a proposed diagnostic approach,” Journal of Ayub Medical College, Abbottabad, vol. 16, no. 1, pp. 8–13, 2004. View at Google Scholar · View at Scopus
  18. M. Imbert, J. Y. Scoazec, J. Y. Mary, H. Jouzult, H. Rochant, and C. Sultan, “Adult patients presenting with pancytopenia: a reappraisal of underlying pathology and diagnostic procedures in 213 cases,” Hematologic Pathology, vol. 3, no. 4, pp. 159–167, 1989. View at Google Scholar · View at Scopus
  19. M. Keisu, W. Heit, G. Lambertenghi-Deliliers, J. Parcells-Kelly, A. Polliack, and H. Heimpel, “Transient pancytopenia. A report from the international agranulocytosis and aplastic study,” Blut, vol. 61, no. 4, pp. 240–244, 1990. View at Publisher · View at Google Scholar · View at Scopus
  20. M. A. Hossain, A. K. Akond, M. K. Chowdhary et al., “Pancytopenia—a study of 50 cases,” Bangladesh Journal of Pathology, vol. 31, no. 1, pp. 9–12, 1992. View at Google Scholar
  21. A. Jha, G. Sayami, R. C. Adhikari, A. D. Panta, and R. Jha, “Bone marrow examination in cases of pancytopenia,” Journal of the Nepal Medical Association, vol. 47, no. 169, pp. 12–17, 2008. View at Google Scholar · View at Scopus
  22. B. M. Mehta, D. V. Rege, and R. S. Satoskar, “Serum vitamin B12 and folic acid activity in lactovegetarian and non vegetarian healthy adult Indians,” The American Journal of Clinical Nutrition, vol. 15, pp. 77–84, 1964. View at Google Scholar
  23. S. J. Thorpe, A. Heath, S. Blackmore et al., “International standard for serum vitamin B12 and serum folate: international collaborative study to evaluate a batch of lyophilised serum for B12 and folate content,” Clinical Chemistry and Laboratory Medicine, vol. 45, no. 3, pp. 380–386, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. Z. Fazili, C. M. Pfeiffer, and M. Zhang, “Comparison of serum folate species analyzed by LC-MS/MS with total folate measured by microbiologic assay and Bio-Rad radioassay,” Clinical Chemistry, vol. 53, no. 4, pp. 781–784, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. J. Lindenbaum, “Status of laboratory testing in the diagnosis of megaloblastic anemia,” Blood, vol. 61, no. 4, pp. 624–627, 1983. View at Google Scholar · View at Scopus
  26. J. Lindenbaum, D. G. Savage, S. P. Stabler, and R. H. Allen, “Diagnosis of cobalamin deficiency—II. Relative sensitivities of serum cobalamin, methylmalonic acid, and total homocysteine concentrations,” American Journal of Hematology, vol. 34, no. 2, pp. 99–107, 1990. View at Publisher · View at Google Scholar · View at Scopus
  27. U. Khanduri and A. Sharma, “Megaloblastic anaemia: prevalence and causative factors,” National Medical Journal of India, vol. 20, no. 4, pp. 172–175, 2007. View at Google Scholar · View at Scopus
  28. U. Khanduri, A. Sharma, and A. Joshi, “Occult cobalamin and folate deficiency in Indians,” National Medical Journal of India, vol. 18, no. 4, pp. 182–183, 2005. View at Google Scholar · View at Scopus
  29. H. Refsum, C. S. Yajnik, M. Gadkari et al., “Hyperhomocysteinemia and elevated methylmalonic acid indicate a high prevalence of cobalamin deficiency in Asian Indians,” American Journal of Clinical Nutrition, vol. 74, no. 2, pp. 233–241, 2001. View at Google Scholar · View at Scopus
  30. J. Selhub, P. F. Jacques, G. Dallal, S. Choumenkovitch, and G. Rogers, “The use of blood concentrations of vitamins and their respective functional indicators to define folate and vitamin B12 status,” Food and Nutrition Bulletin, vol. 29, no. 2, pp. S67–S73, 2008. View at Google Scholar · View at Scopus
  31. J. C. Chambers, O. A. Obeid, H. Refsum et al., “Plasma homocysteine concentrations and risk of coronary heart disease in UK Indian Asian and European men,” Lancet, vol. 355, no. 9203, pp. 523–527, 2000. View at Google Scholar · View at Scopus
  32. R. Sarode, G. Garewal, N. Marwaha et al., “Pancytopenia in nutritional megaloblastic anaemia. A study from north-west India,” Tropical and Geographical Medicine, vol. 41, no. 4, pp. 331–336, 1989. View at Google Scholar · View at Scopus
  33. V. I. Mathan, “Tropical sprue in southern India,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 82, no. 1, pp. 10–14, 1988. View at Google Scholar · View at Scopus