Loss of Ercc1 Results in a Time- and Dose-Dependent Reduction of Proliferating Early Hematopoietic Progenitors
Figure 1
Recombination in Ercc1-flox Tie2-Cre model. The fraction of recombined alleles in the presence of Tie2-Cre was calculated after measuring the nondeleted floxed allele by real-time genomic PCR and comparing it to the presence of the floxed allele in absence of Cre. HPRT was measured to control for total DNA. DNA was isolated from fetal livers at embryonic days E12.5 and E15.5, from the spleen of neonatal mice, and from bone marrow of 10- and 52-week-old mice. Closed triangles: Tie2-Cre; Ercc1+/f, open boxes: Tie2-Cre; Ercc1ā/f. Each symbol is an independent measurement.
