Loss of Ercc1 Results in a Time- and Dose-Dependent Reduction of Proliferating Early Hematopoietic Progenitors

Figure 3

Ercc1 levels influence the composition of the stem and progenitor cell pool. Whole BM suspensions were stained with surface antigen specific antibodies for hematopoietic stem cells. ((a)–(c)) LSK (Lin− Sca1+ cKit+) cells as percentage of total bone marrow cells. ((d)–(f)) Distribution of stem cells within the LSK fraction (LT HSC(CD34− CD135−), ST-HSC (CD34+ CD135−) and MPP (CD34+ CD135+)). ((g)–(i)) Distribution of progenitor cells within total bone marrow (CMP (Lin− cKit+ CD34+ CD16/CD32intermediate), GMP (Lin− cKit+ CD34+ CD16/CD32high), MEP (Lin− cKit+ CD34− CD16/CD32low) and CLP (Lin− CD127+ Sca1/cKit intermediate)). Mean percentages are plotted; error bars indicate standard deviation. *indicates 𝑃≤0.05, **indicates 𝑃≤0.01 and ***indicates 𝑃≤0.001.