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Anemia
Volume 2014, Article ID 640203, 6 pages
http://dx.doi.org/10.1155/2014/640203
Research Article

Correlation between Plasma Interleukin-3, the α/β Globin Ratio, and Globin mRNA Stability

1Biochemistry Department, Pasteur Institute of Iran, Pasteur Street, No. 69, Tehran 1316943551, Iran
2Molecular Medicine Department, Pasteur Institute of Iran, Pasteur Street, No. 69, Tehran 1316943551, Iran
3Immunology Department, Pasteur Institute of Iran, Pasteur Street, No. 69, Tehran 1316943551, Iran
4Department of Epidemiology, Pasteur Institute of Iran, Pasteur Street, No. 69, Tehran 1316943551, Iran

Received 27 August 2014; Accepted 25 October 2014; Published 18 November 2014

Academic Editor: Aurelio Maggio

Copyright © 2014 S. Rouhi Dehnabeh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Globin chain synthesis (GCS) analysis is used in the diagnosis of thalassemia. However, the wide reference range limits its use as a decisive diagnostic tool. It has been shown that α and β  globin mRNA increase through stimulation of cells by interleukin-3 (IL-3). Therefore, this study investigates the relationship between plasma IL-3 and the β/α  globin ratio. Methods. Blood samples were collected from 32 healthy participants on two occasions one month apart. GCS analysis, real-time PCR, and ELISA tests were conducted to determine the β/α  globin ratio, globin mRNA expression and stability rate, and IL-3 levels. Results. On the basis of IL-3 levels, the participants were divided in two groups. One group included participants who showed a significant increase in IL-3 as indicated by a significant rise in mean values of α, β, and γ  globin mRNA, α and β  globin, RBC, and hemoglobin. The other group included participants who showed no difference in IL-3 levels with no significant variations in the above-mentioned parameters. Conclusion. The results of this study indicate that IL-3 has an equivalent positive effect on α and β  globin chain synthesis. Therefore, IL-3 levels do not explain the wide reference range of the α/β  globin ratio.