Aquaculture Nutrition

Research Article

Supplementation of a Multienzyme Complex, an Organic Acid-Essential Oil Complex, and Prebiotic Alone or in Combination Affects Growth, Nutrient Utilization, and Immune Function of Rainbow Trout (Oncorhynchus mykiss)

Table 1

Ingredients and proximate composition of experimental diets (air-dried basis, g/kg).


Items	PC	NC	MC	OEC	P	MC+OEC	MC+P	OEC+P	MC+OEC+P

Fish meal	200.0	100.0	100.0	100.0	100.0	100.0	100.0	100.0	100.0
Soy bean meal	50.0	225.0	225.0	225.0	225.0	225.0	225.0	225.0	225.0
Soy protein concentrate	100.0	100.0	100.0	100.0	100.0	100.0	100.0	100.0	100.0
Wheat flour	259.5	175.9	175.8	175.4	173.9	175.3	173.8	173.4	173.3
Corn gluten meal	50.0	50.0	50.0	50.0	50.0	50.0	50.0	50.0	50.0
Cottonseed protein concentrate	100.0	100.0	100.0	100.0	100.0	100.0	100.0	100.0	100.0
Meat and bone meal	90.0	90.0	90.0	90.0	90.0	90.0	90.0	90.0	90.0
Brewers dried yeast	50.0	50.0	50.0	50.0	50.0	50.0	50.0	50.0	50.0
Fish oil	30.0	37.0	37.0	37.0	37.0	37.0	37.0	37.0	37.0
Soybean oil	30.0	30.0	30.0	30.0	30.0	30.0	30.0	30.0	30.0
DL-methionine	0.0	0.9	0.9	0.9	0.9	0.9	0.9	0.9	0.9
L-lysine	0.0	0.7	0.7	0.7	0.7	0.7	0.7	0.7	0.7
Vitamin premix^a	10.0	10.0	10.0	10.0	10.0	10.0	10.0	10.0	10.0
Mineral premix^b	30.0	30.0	30.0	30.0	30.0	30.0	30.0	30.0	30.0
Y₂O₃	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5
Multienzyme complex	0.0	0.0	0.125	0.0	0.0	0.125	0.125	0.0	0.125
Organic acid-essential oil complex	0.0	0.0	0.0	0.5	0.0	0.5	0.0	0.5	0.5
Prebiotics	0.0	0.0	0.0	0.0	2.0	0.0	2.0	2.0	2.0
Total	1000.0	1000.0	1000.0	1000.0	1000.0	1000.0	1000.0	1000.0	1000.0
Proximate composition
Crude protein	439.1	437.7	437.8	438.5	438.9	436.9	434.3	436.9	436.9
Crude fat	119.2	123.1	125.9	125.4	125.1	120.3	122.0	122.7	121.7
Crude ash	118.9	116.3	114.4	116.9	116.3	117.1	116.7	117.3	114.4
Moisture	86.6	89.7	86.5	83.4	80.3	81.0	82.7	79.9	89.5

^aVitamin premix (mg or IU/kg diet): VA, 10000 IU; VD₃, 3000 IU; VE, 150 IU; VK₃, 12.17 mg; VB₁, 20 mg; VB₂, 20 mg; VB₃, 100 mg; VB₆, 22 mg; VB₁₂, 0.15 mg; VC, 1000 mg; biotin, 0.6 mg; folic acid, 8 mg; inositol, 500 mg. ^bMineral premix(mg/kg diet): iodine, 1.5 mg; cobalt, 0.6 mg; cuprum, 3 mg; iron, 63 mg; manganese, 11.45 mg; selenium, 0.24 mg; magnesium, 180 mg; monocalcium phosphate, 2000 mg.