Involvement of Taste Receptors in the Oro-Sensory Perception of Nutrients in Rainbow Trout (Oncorhynchus mykiss) Fed Diets with Different Fatty Acid Profiles

Table 4

Nucleotide sequences of the PCR primers used to evaluate mRNA abundance of transcripts by RT-PCR (quantitative PCR).

Transcript	Forward primer	Reverse primer	Database	Accession number
Reference
eef1a1	TCCTCTTGGTCGTTTCGCTG	ACCCGAGGGACATCCTGTG	GenBank	AF498320
krt8b	TGGCTACTCCAGTGGTTTCG	CCGCTACCGGAGCTGTAGTT	GenBank	X92522.1
18s	GGCGGCGTTATTCCCATGA	TGCCCTTCCGTCAATTCCTTTA	GenBank	AF309412.1
Neuropeptide
npya	AAGGCAGAGGTGAGTGCTGT	AGCCTGTGGCTCACTAATCAA	GenBanK	NM_001124266
npya	CGTATTTGTTAGCGCCTTTC	TCATTCGATTTCTTCACTTCCA	GenBanK	NM_001124266
agrp1	ACCAGCAGTCCTGTCTGGGTAA	AGTAGCAGATGGAGCCGAACA	GenBanK	CR376289
agrp2	CCAGGAGACGGATTTTGCCA	GAGGGGCACAGCTATCCATC	GenBanK	CA343080
pomca	ACCAGCAGTCCTGTCTGGGTAA	AGTAGCAGATGGAGCCGAACA	GenBanK	NM_001124718.1
pomcb	CCAGGAGACGGATTTTGCCA	GAGGGGCACAGCTATCCATC	GenBanK	NM_001124719.1
cartpt	ACCATGGAGAGCTCCAG	GCGCACTGCTCTCCAA	GenBanK	DQ836925
mc4ra	CCACAACATCATGACCACGC	AGGCAGATGAGGACGGTAGT	GenBanK	XM_021613317.1
mc4rb	CGGTCCTCATCTGCCTCATC	TCCTCTTTATGTGCAGGCGG	GenBanK	XM_021620635.1
Tasting receptors
ffar1	CTCATGGGAAGGCTCTGTGG	CGCCACTCCCCTCACATTAG	Ensembl	ENSOMYG00000041396
ffar2a	GTCCAGTACCATCAACGCCA	CTGCACACTCTCCAACAGGGT	GenBanK	XM_021595180.1
ffar2b/e	CCCATCCAACACTCGCTGAA	TGATGACGACGATGCTCAGG	GenBanK	XM_021595167.1/XM_021561043.1
ffar2c/f	TCATCATCCAGCACCACCAG	GACGAAGAACTCCAGACGCA	GenBanK	XM_021584265.1/XM_021560951.1
ffar2d	TCCGAATCCTCTCTCAGCTG	AGAAGAAGACGCTAGCCACC	GenBanK	XM_021561056.1
ffar2g	TCCTCCTGCTCAACCTGATC	AGGTCATGTTGCAGAGAGGG	GenBanK	XM_021560940.1
ffar3a	TTTTCCACACACAGTTGGCC	AGGTAGTGTTGTCGGCATCT	GenBanK	XM_021571760.1
ffar3b	GTGTGGCCTTCCCTATCAGA	GCAGGGCACAATGTACACAA	GenBanK	XM_021571759.1
gnat2	TCAACGACTCAGCTGGCTAC	GATGATGCCGGTGGTCTTGA	GenBanK	KR999994.1
gpr84	CTCCTCCACCACCTCTTCAG	TCAGCAATGTTCAGCAGCAG	GenBanK	XM_021609929.1
gpr119	GCTACTCCTGATGCACTCCA	CGTAGCGGTCCAGAGAGATC	GenBanK	FR905107.1
cd36	CCTGCTCTCCAAAATCCACG	TATAGTCCCGTTCGCCAGTC	GenBanK	AY606034.1
tas1r1a	GCTGGTGCGTTTTAACTGGA	GGCGATGCAGATGTCAAAGT	GenBanK	XM_021564461.1
tas1r1b	GGGAGCCATCTTCACCAGTA	GCAAGGCCAGGAGATGTTTC	GenBanK	XM_021614421.1
tas1r2	CTGTCGCCATCACACTCATG	GCAGACTTAACAACCGGCTT	GenBanK	FR905259.1
tas1r3	CCAGATACAGAGCCAACCCA	GACCCTGAAAGCTGACCTCT	GenBanK	XM_021569424.1
casr	TGGAAAGTTTGTGTCGGCTG	AGTGTTCCTGGACGGTTTGA	GenBanK	XM_021591413.1
gpr92/93	TGTGGCTGCTTGTGATCAAC	GGGGAACTGGGATTGAAGGA	GenBanK	XM_021613122.1