Involvement of Taste Receptors in the Oro-Sensory Perception of Nutrients in Rainbow Trout (Oncorhynchus mykiss) Fed Diets with Different Fatty Acid Profiles

Table 5

(A) In silico analysis of FFAR1, 2, 3 and 4 identified in rainbow trout (RT) and compared to human, mouse and zebrafish species and identification of sequence homology of FFAR2 (using MUSCLE software) RT paralogs compared to human, mouse and zebrafish FFAR1, FFAR4 (B) or FFAR2 sequence (C).

(a)

	Human (Homo sapiens)	Mouse (Mus musculus)	Zebrafish (danio Reno)	Rainbow trout (Oncorhynchus mykiss)
GPR40 (FFAR1)
GPR43 (FFAR2)				(7 paralogs)
GPR41 (FFAR3)				(2 paralogs
GPR120 (FFAR4)

(b)

	Human (Homo sapiens)	Mouse (Mus musculus)	Zebrafish (danio Reno)
Human FFAR2	100.00	84.55	43.84
Mouse FFAR2	84.55	100.00	45.99
Zebrafish FFAR2	43.83	45.99	100.00
Rainbow trout FFAR2a	47.15	46.20	59.49
Rainbow trout FFAR2b/e	45.43	46.34	57.54
Rainbow trout FFAR2c/f	42.21	41.88	47.23
Rainbow trout FFAR2d	44.88	44.88	47.35
Rainbow trout FFAR2g	45.65	46.27	62.73

(c)

	Human (Homo sapiens)	Mouse (Mus musculus)		Human (Homo sapiens)	Mouse (Mus musculus)
Human FFAR1	100.00	83.00	Human FFAR4	100.00	86.43
Mouse FFAR1	83.00	100.00	Mouse FFAR4	84.55	100.00
Rainbow trout FFAR2a	29.75	28.67	Rainbow trout FFAR2a	19.38	20.49
Rainbow trout FFAR2b/e	30.60	28.93	Rainbow trout FFAR2b/e	21.23	23.04
Rainbow trout FFAR2c/f	31.01	30.48	Rainbow trout FFAR2c/f	20.75	22.4
Rainbow trout FFAR2d	28.39	28.77	Rainbow trout FFAR2d	20.68	21.49
Rainbow trout FFAR2g	28.57	27.18	Rainbow trout FFAR2g	18.09	20.81