Assessment of Performance, Microbial Community, Bacterial Food Quality, and Gene Expression of Whiteleg Shrimp (Litopenaeus vannamei) Reared under Different Density Biofloc Systems

Table 2

Sequences of primers submitted for RT-qPCR to assess immunological, stress, and growth state of shrimp, L. vannamie.

Immune element/stress response and growth	Gene	Abbreviation	PrimerF/R	(5^̀-3̀) primer sequence	References
proPO activating system	Prophenoloxidase	proPO	proPO-F	GAG ATC GCA AGG GAG AAC TG	([77, 87, 88])
			proPO-R	CGT CAG TGA AGT CGA GAC CA
Antimicrobial peptide system	Lysozyme	Lyz	Lyz-F	GAA GCG ACT ACG GCA AGA AC	([87, 88])
			Lyz-R	AAC CGT GAG ACC AGC ACT CT
	Penaiedin4	PEN4	PEN4-F	GCC CGT TAC CCA AAC CAT C	([87, 89])
			PEN4-R	CCG TAT CTG AAG CAG CAA AGT C
Antioxidant defense mechanism	Superoxidase dismutase	SOD	SOD-F	ATC CAC CAC ACA AAG CAT CA	([77, 87–89])
			SOD-R	AGC TCT CGT CAA TGG CTT GT
Pattern recognition receptor	Toll receptor	LvToll	LvToll-F	ATG TGC GTG CGG ATA CAT TA	([77, 87, 88])
			LvToll-R	GGG TGT TGG ATG TCG AGA GT
Stress	Heat shock protein 70 (Lvhsp70)	HSP70	hsp70 -F	GGC AAG GAG CTG AAC AAG TC	([88, 89])
			hsp70 -R	TCT CGA TAC CCA GGG ACA AG
Growth	Ras-related protein rap-2a	Rap-2a	RAP-2a-F	GCC GTG CGT GCT TGA GAT	[86]
			RAP-2a-R	TTG ATG TCC TGG AAG GTC TGG
Internal control	β-Actin		β-Actin-F	CCA CGA GAC CAC CTA CAA C	([77, 89, 90])
			β-Actin-R	AGC GAG GGC AGT GAT TTC