Research Article
Improving Heat Resistance of Nile Tilapia (Oreochromis niloticus) by Dietary Zinc Supplementation
Table 2
Sequences of primers for qRT-PCR analysis.
| | Gene | qPCR primers, forward/reverse (5-3) | GenBank number |
| | β-Actin | F: GGAAATCGTGCGTGACATCA
R: TACCAAGGAAGGAAGGCTGG | XM_003443127 | | HSP70 | F: GCACAAGAAGGACATCAGCC
R: ATCTGCCCCTTGTCCAGTTT | NM_001279671.2 | | IL-10 | F: CTCGCGTCTTTCTTCAGCTC
R: GCAAACGGGCTCTTAAAGGA | KP645180.1 | | TNF-α | F: GCCTCACAATTCTCAGCCAC
R: AAACACGCCAAAGAAGGTCC | AY428948.1 | | INF-γ | F: AAGAATCGCAGCTCTGCACCAT
R: GTGTCGTATTGCTGTGGCTTCC | XM_005448319.1 |
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β-Actin: internal reference gene (housekeeping gene); HSP70: heat shock protein 70; IL-10: interleukin-10; TNF-α: tumor necrosis factor alpha; INF-γ: interferon gamma.
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