Dietary Clostridium butyricum Improves Growth Performance and Resistance to Ammonia Stress in Yellow Catfish (Pelteobagrus fulvidraco)

Table 2

Primers used in real-time PCR.

Target	Forward primer (5-3)	Reverse primer (5-3)	Size (bp)
Claudin-1	ACGCTAACAACGGCTCAGA	CCTTACATTCAGACACCACCTT	171
Zo-1	CGGAGCCACCCAAACAGTAT	TTAGAGTCTCCGCCTCTGCT	129
Occludin	CGAGCGAGAGACTACGACAC	TCCAGGAATTGTGGGCTTCC	239
MLCK	GCCACGAAGACCAAAAGCTC	AACCAAGGGCCAGAGACTTG	118
IL-1	AAACCAGCATCTCCAGTGTC	GAGCAAAGGCTGTTCCGTAT	168
IL-8	CACTCACCAAGCCAGCAATG	AGACAACCCAAGACTTCACC	228
TNF-α	ATAACCCACGCCTATGACTG	GGCTATGACTCGCAACACTT	207
NF-κB	AGCCGTCTTGGCTAACAGTC	AGAGGCGTAGAGCCGTCATA	198
β-Actin	TCCTGACCGAGAGAGGCTAC	TCCAGAGAGGAGGAAGAGGC	136

Zo-1: zonula occludens protein-1; MLCK: myosin light chain kinase; IL-1: interleukin 1; IL-8: interleukin 8; TNF-α: tumor necrosis factor-α; NF-κB: nuclear transcription factor-κB.