Safflower Promotes the Immune Functions of the Common Carp (Cyprinus carpio L.) by Producing the Short-Chain Fatty Acids and Regulating the Intestinal Microflora

Table 2

Primer sequences for qPCR detection genes.

Gene	Accession no.	Forward (5→3)	Reverse (5→3)	Production size (bp)
TNF-α	AJ311800	GTGTCTACAGAAACCCTGGA	AGTAAATGCCGTCAGTAGGA	109
TGF-β	AF136947.1	ACGCTTTATTCCCAACCAAA	GAAATCCTTGCTCTGCCTCA	95
IL-12	AJ621425.1	TCTGTAGAGGTCACATATCCACG	AAGTTCGGTTTGGAGCAGTC	116
IL-10	KX964678.1	CGCCAGCATAAAGAACTCGT	TGCCAAATACTGCTCGATGT	103
IL-6	AY102632	GCAGCGCATCTTGAGTGTTTAC	CTGCTGCTCCATCACTGTCTTC	73
IL-1β	AJ245635	TTACAGTAAGACCAGCCTGA	AGGCTCGTCACTTAGTTTGT	91
NF-κB	AY163837.1	AATGTGGTGCGTCTGTGCTT	TGTTGTCATAGATGGGGTTGGA	100
Occludin	XM042765630.1	TGGGTGAATGATGTGAATGG	ATCCGCTGCTCGGCGGGAC	184
Claudin-3	KF193858	GCCAGATGCAGTGTAAGGTC	CCGTCCAGGAGACAGGGAT	240
TLR-1	LC150761.1	TCGCTTCAATACATCG	AAGGAACTGCGGTCA	190
TLR-4	XM019114012.1	TCATTGTGGTCGTGTCTC	ATGTTGGCATTGCGTTCC	100
18S	FJ710826.1	GAGACTCCGGCTTGCTAAAT	CAGACCTGTTATTGCTCCATCT	107