Supplementing Sulfate-Based Alginate Polysaccharide Improves Pacific White Shrimp (Litopenaeus vannamei) Fed Fishmeal Replacement with Cottonseed Protein Concentrate: Effects on Growth, Intestinal Health, and Disease Resistance
Table 3
Sequence of primers used in this study.
Genes
GenBank no.
Forward (5-3)
Reverse (5-3)
Toll-1
DQ923424
GACCATCCCTTTTACACCAGACT
CCTCGCACATCCAGGACTTTTA
Akirin
KC415269
TCACCGTCTCCATCACCTGC
CAAGCCAATACCTTGTCATACTCC
TRAF6
HM581680
TGGGTCCGTGTCCAGTGAT
ACAAACAACCACACACAAGCAG
ALF
DQ208705
CGCTTCACCGTCAAACCTTAC
GCCACCGCTTAGCATCTTGTT
Crustin
AF430076
CACGAGGCAACCATGAAGG
TCTTGCACCAATACCTGCAGT
LZM
AY170126
TGTTCCGATCTGATGTCC
GCTGTTGTAAGCCACCC
Hsp70
AY645906
ATGGCAAAGGCACCTGCTGT
TGAGCACCATCGAGGAGATCT
SOD
HM371157
AGCCAATGACGTAAGCG
ACCATCACAAGAAACCC
CAT
AY518322
AGAGGGTTGTGCATGCTAAG
CAGCTGATCCACTCTCACCT
β-Actin
AF300705
CCACGAGACCACCTACAACATC
TTAGAAGCACTTCCTGTGAACAATG
β-Actin was set as the reference gene. Abbreviation: TRAF6: TNF receptor associated factor 6; ALF: antilipopolysaccharide factor; LZM: lysozyme; Hsp70: heat shock protein 70; SOD: superoxide dismutase; CAT: catalase.
