Research Article

Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus)

Table 2

Experimental design for the effect of nutrition and scheduled stress (moving net around the tank after the exchange of water on Monday and Friday of proposed weeks) and acute confinement stress (AC stress) in the end of period.

HL, without stressHL, 2-time stressHL, 4-time stressLL, without stressLL, 2-time stressLL, 4-time stress

Week 1Without stressWithout stressWithout stressWithout stressWithout stressWithout stress
Week 2Without stressStressStressWithout stressStressStress
Week 3Without stressWithout stressWithout stressWithout stressWithout stressWithout stress
Week 4Without stressWithout stressStressWithout stressWithout stressStress
Week 5Without stressWithout stressWithout stressWithout stressWithout stressWithout stress
Week 6Without stressWithout stressStressWithout stressWithout stressStress
Week 7Without stressWithout stressWithout stressWithout stressWithout stressWithout stress
Week 8Without stressStressStressWithout stressStressStress
Week 9Without stressWithout stressWithout stressWithout stressWithout stressWithout stress
Calculation of weight of all fish, growth, sampling of blood and other factors
Week 10Without stressWithout stressWithout stressWithout stressWithout stressWithout stress
Final stress (AC stress) and sampling for serum and hematology parameters.