Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus)
Table 2
Experimental design for the effect of nutrition and scheduled stress (moving net around the tank after the exchange of water on Monday and Friday of proposed weeks) and acute confinement stress (AC stress) in the end of period.
HL, without stress
HL, 2-time stress
HL, 4-time stress
LL, without stress
LL, 2-time stress
LL, 4-time stress
Week 1
Without stress
Without stress
Without stress
Without stress
Without stress
Without stress
Week 2
Without stress
Stress
Stress
Without stress
Stress
Stress
Week 3
Without stress
Without stress
Without stress
Without stress
Without stress
Without stress
Week 4
Without stress
Without stress
Stress
Without stress
Without stress
Stress
Week 5
Without stress
Without stress
Without stress
Without stress
Without stress
Without stress
Week 6
Without stress
Without stress
Stress
Without stress
Without stress
Stress
Week 7
Without stress
Without stress
Without stress
Without stress
Without stress
Without stress
Week 8
Without stress
Stress
Stress
Without stress
Stress
Stress
Week 9
Without stress
Without stress
Without stress
Without stress
Without stress
Without stress
Calculation of weight of all fish, growth, sampling of blood and other factors
Week 10
Without stress
Without stress
Without stress
Without stress
Without stress
Without stress
Final stress (AC stress) and sampling for serum and hematology parameters.