Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus)

Table 4

The results of two-way ANOVA with SPSS for measured factors. When the interaction was not significant, we compared the “diet effect” via independent samples -test and “stress-number effect” via Duncan’s new multiple range test; for the case, these effects were significant (). The nonsignificant parameters were not reported. The letters a, b, and c indicate significant differences among treatments exposed to different stress numbers.

	value			Main effects (mean)
	Diet effect	Stress-number effect	Interactions	HL diet	LL diet	0Stress	2Stresses	4Stresses
WG	0.01	0.28	0.70	46.41	38.81
FCR	0.02	0.47	0.22	2.21	2.60
SGR	0.01	0.25	0.82	3.73	3.35
Survival (%) after AC	<0.01	<0.01	0.49	81.25	72.92	62.50^a	90.63^c	78.13^b
Hb	0.02	0.06	0.07	8.60	7.63
BP	0.18	0.01	0.85			64.39^a	63.71^a	59.13^b
Ht-stress	0.06	<0.01	0.07			27.00^b	32.17^a	28.58^b
Hb-stress	0.03	0.02	0.86	7.04	7.92	7.13^b	6.98^b	8.33^a
WBC-stress	0.67	0.02	0.49			18.07^b	21.12^a	18.71^b
MCH-stress	0.01	0.51	0.21	82.66	109.03
MCHC-stress	0.01	<0.01	0.26	23.54	28.35	26.68^a	21.79^b	29.36^a
BP-stress	0.25	<0.01	0.11			56.78^c	64.64^a	60.45^b
TP-stress	0.62	0.02	0.19			3.06^b	3.82^a	3.98^a
Cholesterol	0.01	0.10	0.33	129.99	109.44
Globulin stress	0.49	0.04	0.12			0.93^b	1.11^ab	1.19^a
HDL-stress	0.86	<0.01	0.56			46.18^b	54.82^a	38.20^c
Cholesterol-stress	0.25	<0.01	0.68			99.75^b	127.53^a	100.07^b
Triglyceride-stress	0.53	0.03	0.22			407.10^b	430.50^a	358.60^b
ACH50-stress	0.99	0.02	0.58			132.02^b	160.42^a	151.57^a
Glucose-stress	<0.01	<0.01	0.17	86.83	72.78	94.35^a	73.58^b	71.50^b
Cortisol-stress	0.29	<0.01	0.46			126.10^a	83.87^b	87.55^b
GPx	0.17	0.03	0.35			181.02^b	203.03^ab	215.16^a
SOD-stress	0.04	<0.01	0.10	55.44	63.89	49.03^c	70.37^a	59.58^b
MDA-stress	0.01	0.42	0.56	4.54	5.99
GPx-stress	0.36	<0.01	0.41			149.27^b	200.97^a	180.43^a
ALT	0.65	<0.01	0.74			16.23^a	10.77^b	13.95^a
ALP-stress	0.32	<0.01	0.25			182.81^b	229.38^a	255.82^a
ALT-stress	0.44	<0.01	0.08			23.27^a	11.97^c	17.42^b
AST-stress	0.68	0.03	0.81			233.87^a	191.30^b	199.00^b

“-stress” shows the parameter after acute confinement stress (AC stress). WG: weight gain; FCR: feed conversion ratio; SGR: specific growth rate; Hb: hemoglobin; BP: blood performance; Ht: hematocrit; WBC: white blood cell; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; TP: total protein; HDL: high-density lipoprotein; ACH50: alternative complement activity; GPx: glutathione peroxidase; SOD: superoxide dismutase; MDA: malondialdehyde; ALT: alanine aminotransferase; ALP: alkaline phosphatase; AST: aspartate aminotransferase.