Effects of Dietary Bile Acids on Growth Performance, Lipid Deposition, and Intestinal Health of Rice Field Eel (Monopterus albus) Fed with High-Lipid Diets

Figure 4

Effects of dietary bile acids (BAs) on the mRNA expression related to the intestinal health of rice field eels (Monopterus albus) fed with HLDs. Each data point represents the mean of four replicates (n = 4, mean ± SEM). Means between the CON and the HL groups, means . Bars assigned with different superscripts are significantly different () among the HL, HLLB, and HLHB groups. P_linear means statistical results of orthogonal polynomial contrasts. Intestinal pro-inflammatory factors: tnf-α, il-8, and tlr-8 (a–c), anti-inflammatory factor: il-10 (d), oxidative stress-related genes: nrf2 and keap1 (e, f), intestinal immune barrier-related factors: claudin12 and claudin15 (g, h). tnf-α, tumor necrosis factor alpha; il-8, interleukin-8; tlr-8, toll-like receptor 8; il-10, interleukin-8; nrf2, nuclear factor erythroid 2-related factor 2; keapl, kelch-like ECH associating protein 1; claudin12, CLDN12; claudin15, CLDN15. Treatment groups: CON, control group; HL, high lipid diets group; HLLB, high lipid diets supplementing with 0.025% bile acid group; and HLHB, high lipid diets supplementing with 0.05% bile acid group.

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