Effects of Dietary Bile Acids on Growth Performance, Lipid Deposition, and Intestinal Health of Rice Field Eel (Monopterus albus) Fed with High-Lipid Diets

Table 2

The primer of RT-PCR used in the experiment.

Gene	Forward sequences (5^′⟶3^′)		Reverse sequences (5^′⟶3^′)	Accession no.
tnf-α		TTTCAAGGAGGGCTGGTTCT	CTTGACCAGCGCATCACTGT	XM_020622780.1
il-8		ATGAGTCTTAGAGGTCTGGGT	ACAGTGAGGGCTAGGAGGG	XM_020597092.1
tlr-8		GAGGGCTACGTTAAGACTGGG	GACATTCCTCAGGCTTTGCC	XM_020596483.1
il-10		AATCCCTTTGATTTTGCC	GTGCCTTATCCTACAGTATGTG	XM_020593114.1
nrf2		CTTCAGACAGCGGTGACAGG	GCCTCATTCAGTTGGTGCTT	XM_020608174.1
keap1		AGCCTGGGTGCGATACGA	CAAGAAATGACTTTGGTGGG	XM_020597068.1
claudin12		TCACCTTCAATCGCAACG	ATGTCTGGCTCAGGCTTATCT	XM_020607277.1
claudin15		GGTCTCAGTGTCCTGGTACG	TGGTTTGATGGGACAACGGA	XM_020611334.1
18s r RNA		ATTTCCGACACGGAGAGG	CATGGGTTTAGGATACGCTC	XM_020605951.1

tnf-α, tumor necrosis factor alpha; il-8, interleukin-8; tlr-8, toll-like receptor 8; il-10, interleukin-8; nrf2, nuclear factor erythroid 2-related factor 2; keapl, kelch-like ECH associating protein 1; and 18s r RNA, 18s ribosomal RNA.