Dietary Cholesterol Requirements of Large Red Swamp Crayfish (Procambarus clarkii)

Table 4

Effect of cholesterol level on lipids content of P. clarkii.

Parameters	Dietary cholesterol level (g/kg)						Regression (n = 3)
							ANOVA	Linear		Quadratic
	2.47 (C0)	4.27 (C1)	6.80 (C2)	8.77 (C3)	11.74 (C4)	14.24 (C5)	P value	Adj. R^2	P value	Adj. R^2	P value
Serum
T-CHO (mmol/L)	0.155 ± 0.016^a	0.164 ± 0.017^ab	0.192 ± 0.026^ab	0.201 ± 0.016^ab	0.219 ± 0.035^bc	0.256 ± 0.016^c	0.001	0.731	0.001	0.729	0.001
TG (mmol/L)	0.31 ± 0.02^a	0.38 ± 0.03^ab	0.35 ± 0.08^ab	0.36 ± 0.02^ab	0.45 ± 0.05^b	0.41 ± 0.02^ab	0.02	0.163	0.054	0.16	0.106
HDL-C (mmol/L)	0.152 ± 0.013^a	0.182 ± 0.009^b	0.186 ± 0.006^b	0.205 ± 0.002^c	0.208 ± 0.004^c	0.247 ± 0.004^d	0.001	0.876	0.001	0.873	0.001
LDL-C (mmol/L)	0.099 ± 0.006^a	0.100 ± 0.005^a	0.106 ± 0.003^a	0.118 ± 0.004^b	0.119 ± 0.003^b	0.108 ± 0.005^ab	0.001	0.384	0.004	0.533	0.001
NEFA (mmol/L)	0.12 ± 0.01^bc	0.13 ± 0.01^cd	0.15 ± 0.01^df	0.17 ± 0.00^f	0.11 ± 0.01^ab	0.09 ± 0.01^a	0.001	0.129	0.079	0.701	0.001
Hepatopancreas
T-CHO (mmol/g)	0.326 ± 0.012^a	0.341 ± 0.011^ab	0.345 ± 0.006^ab	0.341 ± 0.005^ab	0.365 ± 0.021^b	0.350 ± 0.013^ab	0.052	0.3	0.011	0.299	0.027
TG (mmol/g)	0.72 ± 0.05^a	0.81 ± 0.07^ab	0.82 ± 0.04^ab	0.88 ± 0.02^b	0.95 ± 0.07^bc	1.01 ± 0.02^c
HDL-C (mmol/g)	0.194 ± 0.008^c	0.162 ± 0.011^ab	0.145 ± 0.013^a	0.141 ± 0.003^a	0.144 ± 0.013^a	0.183 ± 0.001^bc	0.001	0.008	0.301	0.83	0.001
LDL-C (mmol/g)	0.347 ± 0.015^b	0.315 ± 0.020^b	0.309 ± 0.017^b	0.252 ± 0.006^a	0.307 ± 0.020^b	0.325 ± 0.018^b	0.001	0.029	0.238	0.508	0.002
NEFA (μmol/g)	6.44 ± 0.12^a	6.79 ± 0.21^ab	6.93 ± 0.28^ab	6.70 ± 0.33^ab	7.23 ± 0.36^b	6.93 ± 0.14^ab	0.047	−0.061	0.878	−0.122	0.928
Muscle
T-CHO (mmol/g)	0.186 ± 0.019^a	0.201 ± 0.010^a	0.202 ± 0.012^a	0.206 ± 0.010^a	0.212 ± 0.006^a	0.241 ± 0.013^b	0.003	0.595	0.001	0.614	0.001
TG (mmol/g)	0.54 ± 0.04^a	0.57 ± 0.05^a	0.62 ± 0.03^ab	0.64 ± 0.04^ab	0.66 ± 0.03^b	0.71 ± 0.05^b
HDL-C (mmol/g)	0.339 ± 0.023^a	0.368 ± 0.036^ab	0.382 ± 0.014^abc	0.419 ± 0.015^bcd	0.426 ± 0.005^cd	0.439 ± 0.021^c	0.001	0.761	0.001	0.763	0.001
LDL-C (mmol/g)	0.255 ± 0.007^ab	0.251 ± 0.012^ab	0.264 ± 0.005^b	0.263 ± 0.005^b	0.244 ± 0.002^a	0.244 ± 0.020^a	0.006	0.085	0.128	0.317	0.023
NEFA (μmol/g)	0.38 ± 0.02^a	0.41 ± 0.02^a	0.68 ± 0.02^c	0.65 ± 0.01^c	0.65 ± 0.03^c	0.60 ± 0.02^b	0.001	0.103	0.105	0.638	0.001

Note: Values represent mean ± SD. Different letters in the same row indicate a significant difference between groups (). TCHO, total cholesterol; TG, triglyceride; LDL-C, high-density lipoprotein cholesterol; HDL-C, low-density lipoprotein cholesterol; NEFA, nonesterified fatty acids.