Figure 4: Real time qPCR analysis shows that Tq (50 μM) significantly increased p53, p21, and Bax mRNA expression and downregulated Bcl-2 mRNA expression in MiaPaCa-2 cells. Cells were also pretreated with p53 inhibitor, PFT-α, for 1 h prior to addition of Tq. Values are expressed as mean ± SEM of three experiments. versus control levels versus Tq treated values, using one-way repeated ANOVA with subsequent all pairwise comparison procedure by Student’s -test.