Methods to Evaluate the Effect of Ethanol on the Folate Analogue: Fluorescein Methotrexate Uptake in Human Proximal Tubular Cells

Figure 4

Fluoroskan studies of FL-MTX (10 𝜇M) uptake by HPT cells in 24 well plates treated with different concentrations (0, 100, 200, 300, 400, and 500 mg/dL) of ethanol. Ethanol concentration has an impact on FL-MTX uptake. Data are expressed as mean±SEM (𝑛=5).