Advances in Pharmacological and Pharmaceutical Sciences / 2009 / Article / Fig 5

Research Article

Methods to Evaluate the Effect of Ethanol on the Folate Analogue: Fluorescein Methotrexate Uptake in Human Proximal Tubular Cells

Figure 5

Fluoroskan studies of FL-MTX (10  M) uptake by HPT cells in 24 well plates treated with ethanol (500 mg/dL), ET-B receptor antagonist (RES-701-1) (10  M), and PKC selective inhibitor (BIM) (2.5  M). Inhibition of FL-MTX uptake is reversed by the addition of RES-701-1 or BIM. Data are expressed as mean SEM ( ).
291349.fig.005

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