Assessment of 5-HT₇ Receptor Agonists Selectivity Using Nociceptive and Thermoregulation Tests in Knockout versus Wild-Type Mice

Figure 3

Effects of 5-HT₇ receptor agonists AS-19 (a), E-57431 (b), and E-55888 (c) on body temperature in wild-type and 5-HT₇ receptor knockout mice. Subcutaneous administration of AS-19 and E-57431 at 20 mg/kg significantly reduced body temperature in both wild-type and 5-HT₇ receptor knockout mice and showed significant differences between both genotypes. However, E-55888 at 20 mg/kg did not reduce the body temperature neither in wild-type nor in 5-HT₇ knockout mice. Each bar represents the mean ± S.E.M. (𝑛=8–12). ***𝑃<0.001 versus vehicle corresponding group; ^#𝑃<0.05; ^###𝑃<0.001 versus corresponding dose in 5-HT₇ receptor knockout mice (Bonferroni multiple comparison test post-ANOVA).

(a) AS-19

(b) E-5743

(c) E-55888