Allosteric Modulation of Beta1 Integrin Function Induces Lung Tissue Repair
The effect of porcine pancreatic elastase (PPE, 0.2 U/g) on respiratory function in mice and its reversal using the anti-beta1 integrin antibody JB1a (3 mg/kg). (a) The effect of PPE on mean respiratory pressure-volume curves in mice from the 21days (21 d) and 35 days (35 d) after instillation and its reversal by JB1a (vehicle = Veh). (b) Reversal of PPE-induced increase in the quasistatic elastance between 5 and 9 cm H2O by JB1a treatment at different time points after injury. (c) Mean linear intercept (Lm) measurements from the 21 d and 35 d groups. in 35 d groups and in 21 d groups. (d) TUNEL staining demonstrating the effect of JB1a treatment after PPE-induced lung injury. (c) quantification of TUNEL positive cells in lung tissue sections from 21 d and 35 d group following PPE-induced injury and JB1a treatment ( per group). Asterisks denote statistical significance with *, ** and *** in comparison to vehicle.