Figure 3: DHEA induced upregulation of NGF and BDNF facilitated the differentiation of rat cortical neurons. Neurons from the DHEA treated rats were immunostained against neuronal marker MAP-2 (a). The number of neurite extending from each neuron is demonstrated. Anti-NGF and anti-BDNF antibodies (1 μg/mL) were used to block NGF and BDNF action from DHEA treated groups (b). Effects of chronic DHEA treatment on cell proliferation and survival via MTT assay (c). Data represent the mean ± S.D. In each experiment, the data were obtained from nine animals.