Silicone-Acyclovir Controlled Release Devices Suppress Primary Herpes Simplex Virus-2 and Varicella Zoster Virus Infections In Vitro

Figure 3

Comparison of antiviral activity of MED-4750 polymers impregnated with varying quantities of ACV. Vero cells were grown to near-confluence in 24-well culture plates. Implants were prepared by mixing MED-4750 component A, MED-4750 component B, and ACV in varying ratios, and one implant was placed in each well. Two days later, either medium alone (“Mock”) or HSV-2 (G) (95 pfu) was added to the indicated wells. Wells marked “None” received no implant; the rest received implant formulated with the indicated percentage of acyclovir. Wells were photographed 3 days after infection. In the split panel of HSV-2 (G)-infected cells incubated with an implant containing 9% ACV; the top and bottom panels were photographed 3 and 5 days after infection, respectively.