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Advances in Pharmacological Sciences
Volume 2015 (2015), Article ID 714138, 11 pages
Research Article

Olive Mill Waste Extracts: Polyphenols Content, Antioxidant, and Antimicrobial Activities

1Laboratory of Biological Engineering, Faculty of Science and Technologies, Sultan Moulay Slimane University, 23 000 Beni-Mellal, Morocco
2Laboratory of Biochemistry, Faculty of Science, Mohamed First University, 60 000 Oujda, Morocco

Received 30 September 2015; Accepted 9 November 2015

Academic Editor: Ismail Laher

Copyright © 2015 Inass Leouifoudi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Natural polyphenols extracts have been usually associated with great bioactive properties. In this work, we investigated in vitro antioxidant and antimicrobial potential of the phenolic olive mill wastewater extracts (OWWE) and the olive cake extracts (OCE). Using the Folin Ciocalteux method, OWWE contained higher total phenol content compared to OCE ( g/L versus  mg/g). The phenolic compounds identification was carried out with a performance liquid chromatograph coupled to tandem mass spectrometry equipment (HPLC-ESI-MS). With this method, a list of polyphenols from OWWE and OCE was obtained. The antioxidant activity was measured in aqueous (DPPH) and emulsion (BCBT) systems. Using the DPPH assay, the results show that OWWE was more active than OCE and interestingly the extracts originating from mountainous areas were more active than those produced from plain areas (μg/mL; μg/mL, resp.). However, when the antioxidant activity was reversed in the BCBT, OCE produced from plain area was more potent than mountainous OCE. Testing by the gel diffusion assay, all the tested extracts have showed significant spectrum antibacterial activity against Staphylococcus aureus, whereas the biophenols extracts showed more limited activity against Escherichia coli and Streptococcus faecalis.