In Vitro Study on Glucose Utilization Capacity of Bioactive Fractions of Houttuynia cordata in Isolated Rat Hemidiaphragm and Its Major Phytoconstituent

Table 1

Effect of different fractions of H. cordata on glucose utilization by isolated rat hemidiaphragm.

Group	Incubation medium	Glucose uptake (mg/g/30 min)
Control	Tyrode solution with glucose (2%)	15.74 ± 0.38
Insulin	Tyrode solution with glucose (2%) + insulin (0.25 IU/mL)	29.65 ± 0.54
QC	Tyrode solution with glucose (2%) + quercetin (1 mg/mL)	31.42 ± 1.87
Insulin + QC	Tyrode solution with glucose (2%) + insulin (0.25 IU/mL) + quercetin (1 mg/mL)	34.10 ± 1.03
AQ	Tyrode solution with glucose (2%) + aqueous fraction (25 mg/mL)	35.26 ± 0.52
Insulin + AQ	Tyrode solution with glucose (2%) + insulin (0.25 IU/mL) + aqueous fraction (25 mg/mL)	36.21 ± 1.63
EA	Tyrode solution with glucose (2%) + ethyl acetate fraction (25 mg/mL)	24.58 ± 0.56
Insulin + EA	Tyrode solution with glucose (2%) + insulin (0.25 IU/mL) + ethyl acetate fraction (25 mg/mL)	28.60 ± 0.33
CHL	Tyrode solution with glucose (2%) + chloroform fraction (25 mg/mL)	19.32 ± 0.84
Insulin + CHL	Tyrode solution with glucose (2%) + insulin (0.25 IU/mL) + chloroform fraction (25 mg/mL)	23.33 ± 0.54
HEX	Tyrode solution with glucose (2%) + hexane fraction (25 mg/mL)	17.41 ± 0.81
Insulin + HEX	Tyrode solution with glucose (2%) + insulin (0.25 IU/mL) + hexane fraction (25 mg/mL)	22.32 ± 0.36

All results are expressed as mean ± SEM. Statistical comparison was determined by one-way ANOVA followed by Tukey’s multiple comparison test. , statistically significant compared to control group; compared to insulin.
AQ: aqueous fraction, EA: ethyl acetate fraction, CHL: chloroform fraction, and HEX: hexane fraction of H. cordata.