Toxicity and Safety Evaluation of Doxorubicin-Loaded Cockleshell-Derived Calcium Carbonate Nanoparticle in Dogs

Figure 8

Photomicrograph of the longitudinal section of left ventricular myocardium and kidney section. (a) Ventricular myocardium and kidney section of the dogs given normal saline showing normal morphological architecture of the cardiomyocytes, myocardial fibres, and both glomerular (G) and renal tubular structure (PT). (b) Myocardium of the dogs given free DOX 150 mg/m² showing myofibrillar loss, myocardial fibre disruption, multifocal cytoplasmic vacuolation (V), cardiomyocyte disorientation, cellular infiltration (I), and myocytolysis (M) with the kidney section showing dilated glomerular capsular space and dilation of the proximal renal tubular (DPT) with severe intercellular infiltration. (c) Myocardium of the dogs given CS-CaCO₃NP-DOX 250 mg/m² showing myofibrillar loss, vacuolation (v), cardiomyocyte disorientation, and cellular infiltration with the kidney section showing dilated renal tubules and focal areas of congestion. (d) Myocardium of the dogs given CS-CaCO₃NP-DOX 150 mg/m² showing normal myofibril with spindle-shaped centrally placed cardiomyocytes with the kidney section revealing normal architecture glomerulus and renal tubules. (e) Myocardium and kidney section of the dogs given CS-CaCO₃NP-DOX 100 mg/m² showing normal myofibril structure and normal structural morphology of glomerulus and renal tubules (H & E) Scale bar 10 µm.