Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV)

Table 2

Validation of the liquid chromatographic method.

S. no.	Analyte	Retention time	Concentration range (µg/mL)	Calibration equation	Correlation coefficient	Limit of detection (µg/mL)	Limit of quantification (µg/mL)
1	Caffeic acid [1]	5.616	1–20	Y = 8.20e + 004X − 4.64e + 004	0.999836	0.22	0.63
2	Resorcinol [2]	5.745	1–20	Y = 3.66e + 004X − 1.03e + 004	1.000000	0.28	0.66
3	Vanillic acid [6]	6.050	1–20	Y = 1.10e + 005X − 5.72e + 004	0.999948	0.22	0.70
4	p-Coumaric acid [7]	7.563	1–20	Y = 1.88e + 004X − 8.97e + 003	0.999995	0.21	0.66
5	Ferulic acid [9]	8.084	1–20	Y = 1.97e + 004X − 2.17e + 004	0.999669	0.28	0.75
6	Vanillin [10]	8.575	1–20	Y = 1.59e + 004X − 8.61e + 003	0.999997	0.21	0.65
7	Veratric acid [11]	8.899	1–20	Y = 6.32e + 004X − 3.56e + 004	0.999940	0.22	0.71
8	Myristicin [4]	10.061	1–20	Y = 1.14e+ 0 04X − 2.36e + 004	0.997837	0.25	0.72
9	Coumarin [5]	16.501	1–20	Y = 1.67e + 005X + 2.60e + 004	0.999182	0.22	0.69
10	Cinnamic acid	21.630	1–20	Y = 7.73e + 004X − 4.34e + 004	0.999998	0.23	0.67