Evaluation of Anti-HIV-1 Integrase and Anti-Inflammatory Activities of Compounds from Betula alnoides Buch-Ham
Table 3
Anti-inflammatory activity of isolated compounds from B. alnoides in RAW264.7 cells.
Sample
% Inhibition at various concentration (μM)
IC50 (μM)
0
3
10
30
100
Betulinic acid (1)
0.0 ± 3.4
14.6 ± 2.3
22.1 ± 1.2
49.4 ± 3.2
79.5 ± 1.7
31.0 ± 1.9b
Betulin (2)
0.0 ± 3.5
2.9 ± 1.9
13.0 ± 1.6
58.0 ± 1.1
81.8 ± 1.2
30.1 ± 0.3b
Lupeol (3)
0.0 ± 3.5
6.0 ± 1.7
11.4 ± 2.7
23.4 ± 1.9
78.2 ± 1.0
47.3 ± 1.0c
Oleanolic acid (4)
0.0 ± 3.5
10.9 ± 1.8
18.4 ± 1.2
28.2 ± 3.2
63.4 ± 1.0
62.8 ± 3.6d
Ursolic acid (5)
0.0 ± 3.5
5.4 ± 2.3
10.2 ± 3.2
13.6 ± 3.2
67.1 ± 1.8
68.7 ± 5.9d
L-NA
0.0 ± 3.4
19.3 ± 4.7
22.1 ± 1.9
32.6 ± 3.5
79.7 ± 4.8
40.4 ± 6.1bc
Indomethacin
0.0 ± 3.4
32.0 ± 2.6
43.8 ± 1.2
54.1 ± 2.0
79.0 ± 1.4
17.6 ± 1.6a
CAPE
0.0 ± 3.4
22.7 ± 3.1
48.6 ± 0.7
66.8 ± 0.5
85.9 ± 1.0
12.3 ± 0.8a
Each value represents the mean ± S.E.M. of four determinations. Different characters (a, b, c, and d) indicate significant differences among the compared means in the same treatment group at .
