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Advances in Pharmacological Sciences
Volume 2019, Article ID 5420752, 9 pages
Research Article

Analysis of In Vitro Osteoblast Culture on Scaffolds for Future Bone Regeneration Purposes in Dentistry

1Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia
2Basic Oral Research Unit, School of Dentistry, Universidad El Bosque, Bogotá, Colombia
3Department of Physics, School of Sciences, Universidad Nacional de Colombia, Bogotá, Colombia
4Department of Chemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, Colombia

Correspondence should be addressed to Sandra J. Gutiérrez-Prieto; oc.ude.anairevaj@zerreitug.s and Luis Gonzalo Sequeda-Castañeda; oc.ude.anairevaj@adeuqesl

Received 6 June 2018; Revised 18 October 2018; Accepted 30 October 2018; Published 6 February 2019

Guest Editor: Ghulam Hussain

Copyright © 2019 Sandra J. Gutiérrez-Prieto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


One of the main focuses of tissue engineering is to search for tridimensional scaffold materials, complying with nature’s properties for tissue regeneration. Determining material biocompatibility is a fundamental step in considering its use. Therefore, the purpose of this study was to analyze osteoblast cell adhesion and viability on different materials to determine which was more compatible for future bone regeneration. Tridimensional structures were fabricated with hydroxyapatite, collagen, and porous silica. The bovine bone was used as material control. Biocompatibility was determined by seeding primary osteoblasts on each tridimensional structure. Cellular morphology was assessed by SEM and viability through confocal microscopy. Osteoblast colonization was observed on all evaluated materials’ surface, revealing they did not elicit osteoblast cytotoxicity. Analyses of four different materials studied with diverse compositions and characteristics showed that adhesiveness was best seen for HA and viability for collagen. In general, the results of this investigation suggest these materials can be used in combination, as scaffolds intended for bone regeneration in dental and medical fields.