Allergic Airway-Induced Hypersensitivity Is Attenuated by Bergapten in Murine Models of Inflammation

Figure 1

Effect of bergapten on C48/80-induced rat mesenteric mast cell degranulation. A Sprague Dawley rat was sacrificed; pieces of its mesentery excised and treated with either Ringer–Locke (a), normal saline 10 ml kg⁻¹ (b), disodium cromoglycate, DSC 10 μg ml⁻¹ (c), or bergapten 10, 30 and 100 μg ml⁻¹, respectively (d–f). Tissues except the naïve control were challenged with C48/80 (1 μg) for 15 min and stained with Toluidine blue (0.1%, pH 2.5) for 30 min. Representative micrographs of stained mast cells are shown (a–f) and degranulation quantified (g). Data are expressed as mean ± SEM (n = 5). compared to the saline-treated C48/80-challenged group. ^#### compared to naive control group (One-way ANOVA followed by Dunnett’s post hoc test). Micron bar represents 100 μm.

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