Allergic Airway-Induced Hypersensitivity Is Attenuated by Bergapten in Murine Models of Inflammation

Figure 4

Effect of bergapten on lung damage in LPS-induced pulmonary inflammation. C57BL/6 mice were grouped and treated as naïve control, saline 10 ml kg⁻¹ (a), disease control, saline 10 ml kg⁻¹ (b), dexamethasone 10 mg kg⁻¹ (c), or bergapten 3, 10, and 30 mg kg⁻¹ (d–f) for 1 h. Test mice were challenged with LPS while naïve control received PBS only. Mice were sacrificed 24 h later. Lungs were fixed in 10% formalin and embedded in paraffin. 3 μm sections were stained with H&E for histopathological examination. Degree of cell infiltration was quantified using an infiltration score described by Zare et al., (2008) with slight modifications. (g). Data are expressed as mean cell infiltration score ± SEM (n = 5). , , and compared to saline-treated LPS-challenged disease control. ^#### compared to naïve control (one-way ANOVA followed by Dunnett’s post hoc test). Micron bar represents 500 μm.

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