Antioxidant Action and In Vivo Anti-Inflammatory and Antinociceptive Activities of Myrciaria floribunda Fruit Peels: Possible Involvement of Opioidergic System

Table 1

Elution systems, chromogenic agents, and standards used in the phytochemical analysis of extracts from fruit peels of Myrciaria floribunda with thin-layer chromatography (TLC).

Secondary metabolite classes	Standard	Mobile phase	Chromogenic agent
Flavonoids and phenylpropanoids	Quercetin, rutin, and chlorogenic acid	EtOAc-HCOOH-AcOH-H2O (100 : 11 : 11 : 26 v/v)	Natural products-polyethylene glycol reagent
Triterpenes and steroids	β-Sitosterol	Toluene:EtOAc (90 : 10 v/v)	Liebermann–Burchard reagent
Saponins	Escin	EtOAc-HCOOH-AcOH-H2O (100 : 11 : 11 : 26 v/v)	Liebermann–Burchard reagent
Mono and sesquiterpenes	Thymol	Toluene:EtOAc (97 : 3 v/v)	Anisaldehyde-sulfuric acid reagent
Coumarins and quinones	Coumarin and lapachol	CHCl3-MeOH (98 : 2 v/v)	Potassium hydroxide reagent
Alkaloids	Pilocarpine	EtOAc-HCOOH-AcOH-H2O (100 : 11 : 11 : 26 v/v)	Dragendorff reagent
Proanthocyanidins	Catechin	EtOAc-HCOOH-AcOH-H2O (100 : 11 : 11 : 26 v/v)	Vanillin-hydrochloric acid reagent
Hydrolysable tannins	Gallic acid	n-BuOH-H2O-AcOH (40 : 50 : 10 v/v)	Ferric ammonium sulfate 1%