Protective Role of Picralima nitida Seed Extract in High-Fat High-Fructose-Fed Rats

Table 2

Sequences of gene-specific primers.

Gene	Sequence (5′-3′)	Template
GAPDH	Forward AGTGCCAGCCTCGTCTCATA	NM_017008.4
	Reverse GATGGTGATGGGTTTCCCGT
β-Actin	Forward: GTCAGGTCATCACTATCGGCAAT	NM_031144.3
	Reverse: AGAGGTCTTTACGGATGTCAACGT
GK	Forward: CATATGTGCTCCGCAGGACTA	NM_001270850.1
	Reverse: CTTGTACACGGAGCCATCCA
PEPCK	Forward: AGCCTCGACAGCCTGCCCCAGG	NM_198780.3
	Reverse: CCAGTTGTTGACCAAAGGCTTT
HMG-COA reductase	Forward: TGCTGCTTTGGCTGTATGTC	NM_013134.2
	Reverse: TGAGCGTGAACAAGAACCAG
CPT-1α	Forward: AAGTCAACGGCAGAGCAGAG	NM_031559.2
	Reverse: ACGCCCAAGTATTCACAGGG
Leptin	Forward: GCCAAGGCAAACCCATTCTG	XM_008762762.2
	Reverse: GATACCGACTGCGTGTGTGA

GAPDH = Glyceraldehyde 3-phosphate dehydrogenase; GK = glucokinase; PEPCK= Phosphoenolpyruvate carboxykinase; HMG-COA reductase = 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase; CPT-1α = carnitine palmitoyltransferase 1α